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Cell. 1992 Sep 4;70(5):803-10.

New reactions catalyzed by a group II intron ribozyme with RNA and DNA substrates.

Author information

  • 1Institut für Genetik und Mikrobiologie, Universität München, Germany.

Abstract

Here we describe three novel reactions of the self-splicing group II intron bI1 (the first intron of the COB gene of yeast mitochondria) demonstrating its catalytic versatility: reversal of the first step of the self-splicing reaction catalyzed by a linear form of the intron utilizing the energy of a phosphoanhydride bond for transesterification, ligation of a single-stranded DNA to an RNA, and cleavage of a single-stranded DNA substrate. These results have the following evolutionary implications: use of the alpha-beta bond of a terminal triphosphate for transesterification suggests that an RNA RNA replicase could use mononucleotide triphosphates as precursors, and cleavage of single-stranded DNA and DNA-RNA ligation suggests that excised group II introns might integrate directly into DNA without prior reverse transcription.

PMID:
1381286
[PubMed - indexed for MEDLINE]
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