Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also showed decondensation of centromeric heterochromatin. In this instance, however, hypomethylation is detected both in the extended heterochromatin at the centromeres and in the euchromatin of the chromosome arms.