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Biochem Biophys Res Commun. 1992 Aug 14;186(3):1560-6.

Serotonin stimulates a Ca2+ permeant nonspecific cation channel in hepatic endothelial cells.

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  • 1Department of Physiology, Tufts University School of Medicine, Boston, MA 02111.

Abstract

The contraction of hepatic endothelial cell fenestrae after exposure to serotonin is associated with an increase in intracellular Ca2+ which is derived from extracellular Ca2+, is inhibited by pertussis toxin and is not associated with activation of phosphoinositol turnover or cAMP. Using cell-attached and excised patches in primary cultures of rat hepatic endothelial cells, we identified a serotonin-activated channel with conductance of 26.4+/-2.3 pS. The channel was also permeant to Na+, K+ and Ca++ but not to anions. In cell-attached patch recordings, addition of serotonin to the bath significantly increased channel activity with Ca2+ or Na+ as the charge-carrying ions. This channel provides a mechanism whereby serotonin can raise the cytosolic Ca2+ concentration in hepatic endothelial cells.

PMID:
1380808
[PubMed - indexed for MEDLINE]
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