Inhibition of class II MHC gene expression by anti-sense RNA in transgenic mice

Biochem Biophys Res Commun. 1992 Jul 31;186(2):832-7. doi: 10.1016/0006-291x(92)90821-2.

Abstract

We have established transgenic mice carrying the anti-sense DNA to the gene encoding beta chain of the class II major histocompatibility complex (I-A) molecule. The amount of I-A molecule on splenic B lymphocytes from the mice was reduced in the presence of a large amount of the exogenous anti-sense RNA. The amount of I-A beta chain RNA was selectively reduced and inversely correlated with the amount of anti-sense RNA in the spleens. These results suggest that the I-A beta chain RNA is rapidly degraded by duplex formation with the anti-sense RNA in splenic B cells from the transgenic mice.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • DNA, Antisense / genetics*
  • Gene Expression / drug effects
  • Genes, MHC Class II / drug effects*
  • Histocompatibility Antigens Class II / analysis
  • Histocompatibility Antigens Class II / genetics
  • Macromolecular Substances
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • RNA / genetics
  • RNA / isolation & purification
  • RNA Probes
  • RNA, Antisense / genetics
  • RNA, Antisense / pharmacology*
  • Restriction Mapping
  • Ribonucleases
  • Spleen / immunology

Substances

  • Antibodies, Monoclonal
  • DNA, Antisense
  • Histocompatibility Antigens Class II
  • Macromolecular Substances
  • RNA Probes
  • RNA, Antisense
  • RNA
  • Ribonucleases