Abstract
We have established transgenic mice carrying the anti-sense DNA to the gene encoding beta chain of the class II major histocompatibility complex (I-A) molecule. The amount of I-A molecule on splenic B lymphocytes from the mice was reduced in the presence of a large amount of the exogenous anti-sense RNA. The amount of I-A beta chain RNA was selectively reduced and inversely correlated with the amount of anti-sense RNA in the spleens. These results suggest that the I-A beta chain RNA is rapidly degraded by duplex formation with the anti-sense RNA in splenic B cells from the transgenic mice.
MeSH terms
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Animals
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Antibodies, Monoclonal
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DNA, Antisense / genetics*
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Gene Expression / drug effects
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Genes, MHC Class II / drug effects*
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Histocompatibility Antigens Class II / analysis
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Histocompatibility Antigens Class II / genetics
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Macromolecular Substances
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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RNA / genetics
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RNA / isolation & purification
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RNA Probes
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RNA, Antisense / genetics
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RNA, Antisense / pharmacology*
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Restriction Mapping
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Ribonucleases
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Spleen / immunology
Substances
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Antibodies, Monoclonal
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DNA, Antisense
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Histocompatibility Antigens Class II
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Macromolecular Substances
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RNA Probes
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RNA, Antisense
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RNA
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Ribonucleases