Abstract
Neutrophil adherence to endothelium is partially mediated by the expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells activated by agents such as lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). To elucidate molecular mechanisms involved in the induction of ELAM-1 on endothelial cells, we investigated the effect of the NADPH oxidase inhibitor, apocynin (4-hydroxy-3-methoxyacetophenone), on ELAM-1 mRNA expression in human umbilical vein endothelial cells (HUVEC) by Northern blot analysis. Apocynin downregulated both LPS- and PMA-induced ELAM-1 mRNA expression in a dose-dependent manner. Our results suggest NADPH oxidase might play a key role in ELAM-1 mRNA expression in HUVEC.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetophenones / pharmacology*
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Blotting, Northern
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Cell Adhesion Molecules / genetics*
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Cells, Cultured
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E-Selectin
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Gene Expression / drug effects
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Humans
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Lipopolysaccharides
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Membrane Glycoproteins / genetics
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NADH, NADPH Oxidoreductases / antagonists & inhibitors*
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NADPH Oxidases
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology
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Umbilical Veins
Substances
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Acetophenones
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Cell Adhesion Molecules
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E-Selectin
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Lipopolysaccharides
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Membrane Glycoproteins
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RNA, Messenger
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acetovanillone
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NADH, NADPH Oxidoreductases
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NADPH Oxidases
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Tetradecanoylphorbol Acetate