Effect of NADPH oxidase inhibition on endothelial cell ELAM-1 mRNA expression

Biochem Biophys Res Commun. 1992 May 15;184(3):1339-43. doi: 10.1016/s0006-291x(05)80029-4.

Abstract

Neutrophil adherence to endothelium is partially mediated by the expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells activated by agents such as lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). To elucidate molecular mechanisms involved in the induction of ELAM-1 on endothelial cells, we investigated the effect of the NADPH oxidase inhibitor, apocynin (4-hydroxy-3-methoxyacetophenone), on ELAM-1 mRNA expression in human umbilical vein endothelial cells (HUVEC) by Northern blot analysis. Apocynin downregulated both LPS- and PMA-induced ELAM-1 mRNA expression in a dose-dependent manner. Our results suggest NADPH oxidase might play a key role in ELAM-1 mRNA expression in HUVEC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetophenones / pharmacology*
  • Blotting, Northern
  • Cell Adhesion Molecules / genetics*
  • Cells, Cultured
  • E-Selectin
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology*
  • Gene Expression / drug effects
  • Humans
  • Lipopolysaccharides
  • Membrane Glycoproteins / genetics
  • NADH, NADPH Oxidoreductases / antagonists & inhibitors*
  • NADPH Oxidases
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Umbilical Veins

Substances

  • Acetophenones
  • Cell Adhesion Molecules
  • E-Selectin
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • RNA, Messenger
  • acetovanillone
  • NADH, NADPH Oxidoreductases
  • NADPH Oxidases
  • Tetradecanoylphorbol Acetate