Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts

Biochem Biophys Res Commun. 1992 Apr 30;184(2):922-9. doi: 10.1016/0006-291x(92)90679-f.

Abstract

We have isolated and sequenced overlapping cDNA clones for rat KC*. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC*
  • Cloning, Molecular
  • Cytokines / genetics*
  • DNA / genetics*
  • DNA / isolation & purification
  • Fibroblasts / physiology
  • Gene Expression / drug effects
  • Gene Library
  • Growth Substances / genetics*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-1 / pharmacology
  • Macrophages, Alveolar / physiology*
  • Molecular Sequence Data
  • Multigene Family
  • Oligonucleotide Probes
  • Platelet Factor 4 / genetics*
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / genetics*
  • RNA-Directed DNA Polymerase
  • Rats
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC
  • Cxcl1 protein, mouse
  • Cxcl1 protein, rat
  • Cytokines
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-1
  • Oligonucleotide Probes
  • RNA, Messenger
  • Recombinant Proteins
  • keratinocyte-derived chemokines
  • Platelet Factor 4
  • DNA
  • RNA-Directed DNA Polymerase

Associated data

  • GENBANK/D10556
  • GENBANK/M86536
  • GENBANK/M97582
  • GENBANK/M97583
  • GENBANK/M97584
  • GENBANK/M97585
  • GENBANK/M97586
  • GENBANK/M97587
  • GENBANK/M97588
  • GENBANK/M97589