Abstract
We have isolated and sequenced overlapping cDNA clones for rat KC*. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cell Line
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Cells, Cultured
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Chemokine CXCL1
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Chemokines
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Chemokines, CXC*
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Cloning, Molecular
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Cytokines / genetics*
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DNA / genetics*
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DNA / isolation & purification
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Fibroblasts / physiology
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Gene Expression / drug effects
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Gene Library
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Growth Substances / genetics*
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Humans
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Intercellular Signaling Peptides and Proteins*
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Interleukin-1 / pharmacology
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Macrophages, Alveolar / physiology*
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Molecular Sequence Data
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Multigene Family
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Oligonucleotide Probes
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Platelet Factor 4 / genetics*
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Polymerase Chain Reaction / methods
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RNA, Messenger / genetics*
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RNA-Directed DNA Polymerase
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Rats
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Recombinant Proteins / pharmacology
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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CXCL1 protein, human
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Chemokine CXCL1
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Chemokines
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Chemokines, CXC
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Cxcl1 protein, mouse
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Cxcl1 protein, rat
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Cytokines
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-1
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Oligonucleotide Probes
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RNA, Messenger
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Recombinant Proteins
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keratinocyte-derived chemokines
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Platelet Factor 4
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DNA
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RNA-Directed DNA Polymerase
Associated data
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GENBANK/D10556
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GENBANK/M86536
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GENBANK/M97582
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GENBANK/M97583
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GENBANK/M97584
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GENBANK/M97585
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GENBANK/M97586
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GENBANK/M97587
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GENBANK/M97588
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GENBANK/M97589