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An acidic fibroblast growth factor protein generated by alternate splicing acts like an antagonist.
Department of Microbiology, University of California School of Medicine, San Francisco 94143.
Polymerase chain reaction amplification of cDNA for acidic fibroblast growth factor in several lines of cultured human cells revealed two forms of mRNA. The novel smaller mRNA lacks the entire second coding exon of the acidic fibroblast growth factor gene, whereas the previously identified mRNA consists of three coding exons. The truncated variant of acidic fibroblast growth factor (aFGF') is only 60 amino acids long with an apparent molecular mass of 6.7 kD on sodium dodecyl sulfate gels in contrast to 18 kD for the full-length acidic fibroblast growth factor. aFGF' elicits only minimal fibroblast proliferation and antagonizes the effects of acidic fibroblast growth factor when added exogenously to or when coexpressed with aFGF in BALB/c/3T3 fibroblasts. Thus, the truncated variant of acidic fibroblast growth factor may provide fibroblasts with a unique mechanism for endogenous regulation of their responses to acidic fibroblast growth factor.
PMID: 1372643 [PubMed - indexed for MEDLINE]
PMCID: PMC2119192
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Cited by 3 PubMed Central articles
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Expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (FGFR4) in breast fibroadenomas.
La Rosa S, Sessa F, Colombo L, Tibiletti MG, Furlan D, Capella C.
J Clin Pathol. 2001 Jan; 54(1):37-41.
[J Clin Pathol. 2001]
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Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development.
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Proc Natl Acad Sci U S A. 1996 Sep 3; 93(18):9850-7.
[Proc Natl Acad Sci U S A. 1996]
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Stimulation of proliferation of a human osteosarcoma cell line by exogenous acidic fibroblast growth factor requires both activation of receptor tyrosine kinase and growth factor internalization.
Wiedłocha A, Falnes PO, Rapak A, Muñoz R, Klingenberg O, Olsnes S.
Mol Cell Biol. 1996 Jan; 16(1):270-80.
[Mol Cell Biol. 1996]