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Bioprocess Biosyst Eng. 2003 Jul;25(6):341-8. Epub 2003 Apr 11.

kLa determination: comparative study for a gas mass balance method.

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  • 1Laboratoire de Génie Chimique et Biochimique, Université Blaise Pascal, CUST, 24 avenue des Landais, BP 206, 63174 Aubière-Cédex, France. laurent.poughon@univ-bpclermont.fr

Abstract

The determination of k(L) a by a gas balance method coupled with sulphite oxidation is compared for three kinds of processes (stirred tank, bubble column and fixed-bed column reactors) with a gassing-in and with a classical chemical sulphite oxidation method. The mathematical relations required for the determination of the k(L) a value are detailed. In coalescing gas-liquid conditions, the values calculated by the three methods are shown to be comparable. The gas balance method is more rapid than either the steady-state gassing-in or the chemical sulphite reaction rate measurement methods. It is also well adapted for three-phase systems (gas-liquid-solid) in which the non-coalescing effects of sulphite solution are reduced by solid interferences.

PMID:
13680342
[PubMed]
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