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Strategies for improving plasmid stability in genetically modified bacteria in bioreactors.
Institut für Technische Chemie, Universität Hannover, FRG.
Exploitation of recombinant organisms for the large-scale, commercial production of foreign proteins is often hampered by the problem of plasmid instability. A wide range of strategies have been reported for improving the stability of recombinant organisms. A combination of manipulating both the genetic design of recombinants and the conditions of culturing the organisms may be used to achieve stable host-vector associations during culture of recombinant organisms in bioreactors.
PMID: 1367568 [PubMed - indexed for MEDLINE]
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Cited by 2 PubMed Central articles
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Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli.
Sletta H, Nedal A, Aune TE, Hellebust H, Hakvåg S, Aune R, Ellingsen TE, Valla S, Brautaset T.
Appl Environ Microbiol. 2004 Dec; 70(12):7033-9.
[Appl Environ Microbiol. 2004]
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Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications.
Panke S, de Lorenzo V, Kaiser A, Witholt B, Wubbolts MG.
Appl Environ Microbiol. 1999 Dec; 65(12):5619-23.
[Appl Environ Microbiol. 1999]