Neurochem Int. 1992 Mar;20 Suppl:165S-170S.

The phosphorylation state of DARPP-32, a third messenger for dopamine, is regulated by in vivo pharmacological treatments.

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Institute of Pharmacological Sciences, University of Milano, Italy.

Abstract

It has been recently proposed that DARPP-32 participates, as third messenger, in the mediation of effects induced by dopamine at the cellular level. DARPP-32 is indeed localized almost exclusively on dopaminoceptive neurons bearing the D1 receptor subtype and it is phosphorylated by cAMP-dependent protein kinase. In its phospho-form, DARPP-32 acts as an inhibitor of protein phosphatase-1. In vivo pharmacological treatment with selective D1 agonists and antagonists induces changes in the phosphorylation state of DARPP-32 that can be correlated to changes in cAMP, mediated in turn by D1 and D2 receptors. These data demonstrate that the measurement of the phosphorylation state of DARPP-32 with the back-phosphorylation assay can represent a useful biochemical tool to gain further insight into the sequence of events elicited by specific dopaminergic drugs in vivo.

PMID:: 1365418; [PubMed - indexed for MEDLINE]

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