Abstract
Regulation of the gene, fimA, encoding the major fimbrial subunit of S. typhimurium S6704 was examined by using a lambda fimA-lacZ lysogen. Transformation of the lambda fimA-lacZ lysogen with various derivatives of the recombinant plasmid that encodes type 1 fimbrial expression, pISF101, indicated that two regions of this plasmid alter beta-galactosidase production. One plasmid is a deletion resulting in the loss of a 28-kDa polypeptide downstream of fimA, while the other plasmid encodes a 24- and a 27-kDa polypeptide. Northern (RNA) blot analyses indicated that the steady-state fimA mRNA levels of these transformants were high. In addition, phenotypic expression of type 1 fimbriae by agar-grown cultures is observed only in those transformants bearing plasmids which show increased beta-galactosidase and fimA mRNA levels.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Base Sequence
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DNA Mutational Analysis
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DNA-Binding Proteins
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Fimbriae Proteins*
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Fimbriae, Bacterial / physiology*
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial / genetics*
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Membrane Proteins
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Molecular Sequence Data
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RNA, Messenger / biosynthesis
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Recombinant Fusion Proteins
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Salmonella typhimurium / genetics*
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Transformation, Genetic
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Membrane Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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fimbrillin
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Fimbriae Proteins
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beta-Galactosidase
Associated data
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GENBANK/L01135
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GENBANK/L01136
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GENBANK/L01137
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GENBANK/L01138
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GENBANK/L01139
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GENBANK/L01140
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GENBANK/L01141
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GENBANK/M90677
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GENBANK/M98391
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GENBANK/Z11768