A comparison of methods for preparing a jellyfish nematocyst suspension from sea nettle (Chrysaora quinquecirrha) fishing tentacles at the beachside was conducted. Autolysis of the tentacle followed by straining and sedimentation on ice was found to be a satisfactory technique. This procedure utilized a tea strainer, plastic cup and conical centrifuge tube, all of which could be made available at a minimally equipped laboratory.