J Mol Biol. 1992 Jun 20;225(4):933-8.

From adenylate cyclase to guanylate cyclase. Mutational analysis of a change in substrate specificity.

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Institut Pasteur, Paris, France.

Abstract

Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was selected displaying a significant level of guanylate cyclase activity. This was due to a point mutation destroying most of the adenylate cyclase activity. A second selection step restored most of the original activity. This resulted from an additional mutation in the same region, thus permitting the first identification of a functional domain in adenylate and guanylate cyclases.

PMID:: 1351950; [PubMed - indexed for MEDLINE]

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From adenylate cyclase to guanylate cyclase. Mutational analysis of a change in ...
From adenylate cyclase to guanylate cyclase. Mutational analysis of a change in substrate specificity.
J Mol Biol. 1992 Jun 20 ;225(4):933-8.

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