Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types

J Gen Virol. 1992 Dec:73 ( Pt 12):3263-8. doi: 10.1099/0022-1317-73-12-3263.

Abstract

DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cervix Uteri / microbiology
  • DNA, Viral / analysis*
  • DNA, Viral / genetics
  • Female
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Papanicolaou Test
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Polymerase Chain Reaction
  • Tumor Virus Infections / diagnosis*
  • Tumor Virus Infections / genetics
  • Vaginal Smears

Substances

  • DNA, Viral
  • Oligodeoxyribonucleotides