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J Virol Methods. 1992 Sep;39(3):279-89.

Examination of the buoyant density of hepatitis C virus by the polymerase chain reaction.

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  • 1Abbott Laboratories, North Chicago, IL 60064.


Sucrose and cesium chloride density gradients were used to fractionate hepatitis C virus (HCV) infectious chimpanzee plasma. The fractionated plasma was then evaluated for HCV RNA sequences using cDNA synthesis and the polymerase chain reaction (cDNA/PCR). cDNA/PCR detectable HCV RNA was identified repeatedly in two regions. One region was at the top of the gradients with a buoyant density of < or = 1.03 g/cm3, the other at a density of approximately 1.18-1.21 g/cm3.

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