Effect of interleukin-3 and granulocyte-macrophage colony-stimulating factor on growth of xenotransplanted human tumour cell lines in nude mice

Eur J Cancer. 1992;28(2-3):377-80. doi: 10.1016/s0959-8049(05)80057-7.

Abstract

The clonal growth of cell lines from some human solid tumours can be stimulated by haematopoietic growth factors such as recombinant human (rh) interleukin-3 (IL-3) and rh granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro. Among these cell lines are the human colorectal adenocarcinoma cell line HTB 38 and the human small-cell lung cancer cell line HTB 119. Here we report on a series of experiments studying the influence of subcutaneously administered rhIL-3 and rhGM-CSF on the in vivo growth of HTB 38 and HTB 119 cell lines as xenografts in athymic nu/nu BALB/c mice. Beginning 1 day after transplantation of the tumour the cytokines were administered daily for 20 days as a subcutaneous bolus distant from the tumour lesion at dose levels up to 1 mg/m2/day. The cytokines caused no significant and reproducible growth modulation of the tumours in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / pathology*
  • Animals
  • Carcinoma, Small Cell / pathology*
  • Cell Division / drug effects
  • Cell Line
  • Colorectal Neoplasms / pathology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
  • Humans
  • Interleukin-3 / pharmacology*
  • Lung Neoplasms / pathology*
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Recombinant Proteins / pharmacology
  • Transplantation, Heterologous

Substances

  • Interleukin-3
  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor