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EMBO J. 1992 Mar;11(3):1145-53.

Demonstration of retrotransposition of the Tf1 element in fission yeast.

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  • 1Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205.

Abstract

Tf1, a retrotransposon from fission yeast, has LTRs and coding sequences resembling the protease, reverse transcriptase and integrase domains of retroviral pol genes. A unique aspect of Tf1 is that it contains a single open reading frame whereas other retroviruses and retrotransposons usually possess two or more open reading frames. To determine whether Tf1 can transpose, we overproduced Tf1 transcripts encoded by a plasmid copy of the element marked with a neo gene. Approximately 0.1-4.0% of the cell population acquired chromosomally inherited resistance to G418. DNA blot analysis demonstrated that such strains had acquired both Tf1 and neo specific sequences within a restriction fragment of the same size; the size of this restriction fragment varied between different isolates. Structural analysis of the cloned DNA flanking the Tf1-neo element of two transposition candidates with the same regions in the parent strain showed that the ability to grow on G418 was due to transposition of Tf1-neo and not other types of recombination events.

PMID:
1312461
PMCID:
PMC556557
[PubMed - indexed for MEDLINE]
Free PMC Article
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