Nat Struct Biol. 2003 Oct;10(10):856-63. Epub 2003 Aug 31.

Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains.

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Howard Hughes Medical Institute and Department of Molecular and Cellular Physiology, Stanford University, James H. Clark Center E300-C, 318 Campus Drive, Stanford, California 94305-5432, USA.

Abstract

The ATPase p97/VCP affects multiple events within the cell. These events include the alteration of both nuclear and mitotic Golgi membranes, the dislocation of ubiquitylated proteins from the endoplasmic reticulum and regulation of the NF-kappa b pathway. Here we present the crystal structure of full-length Mus musculus p97/VCP in complex with a mixture of ADP and ADP-AlF(x) at a resolution of 4.7 A. This is the first complete hexameric structure of a protein containing tandem AAA (ATPases associated with a variety of cellular activities) domains. Comparison of the crystal structure and cryo-electron microscopy (EM) reconstructions reveals large conformational changes in the helical subdomains during the hydrolysis cycle. Structural and functional data imply a communication mechanism between the AAA domains. A Zn(2+) occludes the central pore of the hexamer, suggesting that substrate does not thread through the pore of the molecule.

PMID:: 12949490; [PubMed - indexed for MEDLINE]

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Cited by 35 PubMed Central articles

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Structures reported by this article

Structure Of P97VCP IN COMPLEX WITH ADPAMP-Pnp

PDB: 3CF2

Source: Mus musculus

Method: X-Ray Diffraction

Resolution: 3.5 Å

See all 2 structures...

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Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains.

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