Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biotechnol. 2003 Sep 4;104(1-3):287-99.

Tools for genetic engineering in the amino acid-producing bacterium Corynebacterium glutamicum.

Author information

  • 1Lehrstuhl für Gentechnologie/Mikrobiologie, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany.

Abstract

During the last decades, the gram-positive soil bacterium Corynebacterium glutamicum has been shown to be a very versatile microorganism for the large-scale fermentative production of L-amino acids. Up to now, a vast amount of techniques and tools for genetic engineering and amplification of relevant structural genes have been developed. The objectives of this study are to summarize the published literature on tools for genetic engineering in C. glutamicum and to focus on new sophisticated and highly efficient methods in the fields of DNA transfer techniques, cloning vectors, integrative genetic tools, and antibiotic-free self-cloning. This repertoire of C. glutamicum methodology provides an experimental basis for efficient genetic analyses of the recently completed genome sequence.

PMID:
12948646
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk