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Theriogenology. 2003 Oct 1;60(6):1127-38.

Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry.

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  • 1Department of Physiology and Pharmacology, Texas A&M University, College of Veterinary Medicine, College Station, TX 77843-4475, USA.


Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and propidium iodide (PI) while the second protocol added the mitochondrial probe JC-1. The relationship between total sperm motility and percent viable sperm was high between staining protocols (r = 0.98). Time (0 h versus 24 h, P<0.0001) and treatment (0, 10, 25, 50, and 75% nonviable sperm, P<0.0001) affected percent total sperm motility and percent viable sperm for both staining protocols. Actual percent viable sperm for each time and treatment did not differ from expected values.

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