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Extremophiles. 2003 Aug;7(4):291-8. Epub 2003 Apr 9.

Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae.

Author information

  • 1Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Central-6, Higashi 1-1, Tsukuba 305-8566, Ibaraki, Japan. hitoshi.iwahashi@aist.go.jp

Erratum in

  • Extremophiles. 2003 Oct;7(5):433.

Abstract

Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few hundred MPa decreases the viability of yeast cells, and pressure of a few tens MPa decreases the growth rate. To understand the effect of hydrostatic pressure, we employed yeast DNA microarrays and analyzed genome-wide gene-expression levels after the pressure treatment with 180 MPa (immediate) at 4 degrees C and recovery incubation for 1 h and 40 MPa (16 h) at 4 degrees C and recovery incubation for 1 h. The transcription of genes involved in energy metabolism, cell defense, and protein metabolism was significantly induced by the pressure treatment. Genome-wide expression profiles suggested that high pressure caused damage to cellular organelles, since the induced gene products were localized in the membrane structure and/or cellular organelles. Hierarchical clustering analysis suggested that the damage caused by the pressure was similar to that caused by detergents, oils, and freezing/thawing. We also estimated the contribution of induced genes to barotolerance using some strains that have the deletion in the corresponding genes.

PMID:
12910389
[PubMed - indexed for MEDLINE]
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