Quantification analysis of translation initiation signal in vertebrate mRNAs: effect of nucleotides at positions +4(-)+6 upon efficiency of translation initiation

Nucleic Acids Symp Ser. 2000:(44):77-8. doi: 10.1093/nass/44.1.77.

Abstract

Concerning the translation initiation signal in vertebrate mRNAs, a consensus, sequence, (GCC)GCC(A or G) CCATGG, has been proposed, but actual initiation sequences differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a function of mutations introduced at positions +4, and +6 of the first ATG codon. Codons possessing G at +4 strongly enhanced selection of the first ATG codon. However, ATG codon recognition was unaffected by most mutations in positions and +6. These data were well understood by our quantification analysis.

MeSH terms

  • Animals
  • Base Sequence
  • Codon, Initiator / genetics
  • Mutation
  • Peptide Chain Initiation, Translational*
  • RNA, Messenger / genetics*
  • Ribosomes / metabolism

Substances

  • Codon, Initiator
  • RNA, Messenger