Synthesis and application of bis (tert-butyl)-1-methyl pyrenyl-silyl chloride as a 5'-hydroxyl protecting group for nucleosides and as a fluorescent purification handle in oligonucleotide synthesis

Snehlata Tripathi; Krishna Misra; Yogesh S Sanghvi

doi:10.1093/nass/2.1.117

Synthesis and application of bis (tert-butyl)-1-methyl pyrenyl-silyl chloride as a 5'-hydroxyl protecting group for nucleosides and as a fluorescent purification handle in oligonucleotide synthesis

Nucleic Acids Res Suppl. 2002:(2):117-8. doi: 10.1093/nass/2.1.117.

Authors

Snehlata Tripathi¹, Krishna Misra, Yogesh S Sanghvi

Affiliation

¹ Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211002, India.

PMID: 12903133
DOI: 10.1093/nass/2.1.117

Abstract

A fluorescently labelled monomer viz. 5'-O-1, 1-bis-(tert-butyl)-1'-methyl-pyrenyl-silyl-thymidine-3'-phosphoramidite was synthesized. Different sequences were synthesized and were labelled using it and their fluorescence studies were carried out.

MeSH terms

Base Sequence
Fluorescent Dyes / chemistry*
Nucleosides / chemistry*
Oligonucleotides / chemical synthesis*
Pyrenes / chemistry*
Thymidine / analogs & derivatives
Thymidine / chemistry*

Substances

5'-O-1,1-bis(tert-butyl)-1'-methylpyrenylsilylthymidine-3'-phosphoramidite
Fluorescent Dyes
Nucleosides
Oligonucleotides
Pyrenes
Thymidine