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Biochem Biophys Res Commun. 2003 Aug 22;308(2):313-9.

Myelin basic protein has multiple calmodulin-binding sites.

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  • 1Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ont., Canada N1G 2W1.

Abstract

Myelin basic protein (MBP) has been shown to bind calmodulin (CaM) in a specific Ca(2+)-dependent manner via a primary target sequence at its C-terminus [Protein Sci. 12 (2003) 1507]. Upon deimination of MBP, the nature of the interaction changed significantly, suggesting either a new binding site or different conformers with different affinities for CaM. In order to resolve this issue, we investigated here the CaM-binding properties of N- and C-terminal deletion mutants of MBP using Trp fluorescence spectroscopy and mass spectrometry. We conclude that there is an additional CaM-binding site on MBP in a central segment (we posit murine residues 82-93) that forms an amphipathic alpha-helix.

PMID:
12901870
[PubMed - indexed for MEDLINE]
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