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J Bacteriol. 2003 Aug;185(16):5012-4.

Regulated expression of the Escherichia coli dam gene.

Author information

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.

Abstract

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

PMID:
12897023
[PubMed - indexed for MEDLINE]
PMCID:
PMC166483
Free PMC Article

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