[Properties of skeletal muscle sarcoplasmic reticulum membranes prepared in the presence of dithiothreitol]

Ustav lekárskej biochémie Jeseniovej LFUK, Martine, CSFR.

The authors studied the effect of dithiothreitol (DTT), an agent modulating the redox state of SH-groups, on functional properties of sarcoplasmic reticulum membranes of skeletal muscle. DTT (5 mmol.l-1) exerted a protective effect not only on (Ca2 + Mg2)-ATPase activity but also on the activity of Ca2+ uptake by vesicles, while the most pronounced effect was recorded at low levels of ionized Ca2+. The presence of DTT in the isolation medium decreased also passive permeability of membranes for Ca2+. In the light of the results obtained, modulation of the redox state is recommended for each isolation of membranes designed for the study of ion transport.

PMID: 1288820 [PubMed - indexed for MEDLINE]