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Appl Biochem Biotechnol. 1992 Nov;37(2):201-7.

Synergism between Clostridium thermocellum cellulases cloned in Escherichia coli.

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  • 1Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.


We have obtained a synergistic effect during degradation of Avicel and filter paper by Clostridium thermocellum cellulases (two endoglucanases and one cellobiohydrolase) cloned in Escherichia coli. The highest degree of synergism was found at early stages of reaction, during the first 20 h: 2.5 and 2.9 on Avicel and filter paper, respectively. During combined action of all three cellulases the main product is cellobiose.

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