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    Blood. 2003 Nov 1;102(9):3238-40. Epub 2003 Jul 17.

    The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish.

    Ward AC, McPhee DO, Condron MM, Varma S, Cody SH, Onnebo SM, Paw BH, Zon LI, Lieschke GJ.

    Source

    Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood, Victoria, Australia. award@deakin.edu.au

    Abstract

    The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.

    PMID:
    12869502
    [PubMed - indexed for MEDLINE]
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