The cyclin in the RNA polymerase holoenzyme is a target for the transcriptional repressor Tup1p in Saccharomyces cerevisiae

J Mol Microbiol Biotechnol. 2003;5(4):199-205. doi: 10.1159/000071071.

Abstract

The general transcriptional repressor Tup1p requires the cyclin/cyclin-dependent kinase pair Srb11p/Srb10p in the holoenzyme of transcription. We used the split-ubiquitin system to demonstrate that Tup1p interacts with Srb11p in vivo. We confirmed our observation in vitro with the help of purified proteins, and we compared the de-repression effect of deleting TUP1, SRB10, and SRB11 on different promoters. We propose that Tup1p targets the cyclin Srb11p to affect the cyclin-dependent kinase Srb10p.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / metabolism*
  • DNA-Directed RNA Polymerases / metabolism*
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic
  • Protein Binding
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Transcription Factors
  • Transcription, Genetic

Substances

  • Cyclins
  • Nuclear Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • TUP1 protein, S cerevisiae
  • Transcription Factors
  • SSN8 protein, S cerevisiae
  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases
  • SSN3 protein, S cerevisiae
  • DNA-Directed RNA Polymerases