Abstract
Interleukin-1 (IL-1) receptor-associated kinases (IRAKs) are central components of Toll/IL-1 receptor (TIR) signaling pathways. In an attempt to discover novel signal transducers in TIR signaling, we identified human Pellino2 as an interaction partner of IRAK4. Pellino2 interacts with kinase-active as well as kinase-inactive IRAK1 and IRAK4. Furthermore, Pellino2 is one of the first substrates identified for IRAK1 and IRAK4. Functional studies using overexpression or RNAi knock-down of Pellino2 suggest a role of Pellino2 as a scaffolding protein similar to Pellino1. However, unlike Pellino1, Pellino2 does not seem to activate a specific transcription factor, but links TIR signaling to basic cellular processes.
MeSH terms
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Cell Line
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Cells, Cultured
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Cloning, Molecular
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Genes, Reporter
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Genetic Vectors
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Humans
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Interleukin-1 Receptor-Associated Kinases
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Substrate Specificity
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Ubiquitin-Protein Ligases
Substances
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Nuclear Proteins
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Recombinant Proteins
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PELI1 protein, human
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Ubiquitin-Protein Ligases
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Protein Kinases
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Phosphotransferases (Alcohol Group Acceptor)
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IRAK1 protein, human
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IRAK4 protein, human
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Interleukin-1 Receptor-Associated Kinases