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FEBS Lett. 2003 Jul 17;547(1-3):115-8.

Specific covalent binding of a NF-kappaB decoy hairpin oligonucleotide targeted to the p50 subunit and induction of apoptosis.

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  • 1ATHSCO EA3406 Université Paris XIII, Service de Biochimie APHP Hôpital Avicenne, 125 route de Stalingrad, 93009 Cedex, Bobigny, France.

Abstract

The NF-kappaB transcriptional factor regulates various functions such as immune responses, cellular growth and development, and is frequently activated in tumor cells. Thus, inhibition of NF-kappaB could suppress tumor cell growth. Using a decoy synthetic hairpin-shaped oligodeoxyribonucleotide (ODN) containing the kappaB site with an integrated single diphosphoryldisulfide linkage, we demonstrate its covalent binding to the p50 subunit of NF-kappaB. Furthermore, this decoy ODN induces apoptosis in a lymphoblastoma cell line. Thus, such chemically modified decoys could be valuable tools for blocking nuclear factors and tumor cell growth.

PMID:
12860397
[PubMed - indexed for MEDLINE]
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