The final two steps in the biosynthetic pathway of CoA. CoA biosynthesis occurs in a series of five steps that utilize pantothenate (vitamin B₅), cysteine, and ATP. PPAT catalyzes the penultimate step, the reversible transfer of an adenylyl group from ATP to 4′-phosphopantetheine to form dPCoA and pyrophosphate. Subsequent phosphorylation at the 3′-hydroxyl of the ribose ring by dPCoA kinase produces the acyl group carrier, CoA. For CoA, the 3′-phosphate ADP is shown by a dashed box, the pantothenic acid group of pantetheine is outlined by the solid box, and the β-mercaptoethylamine of pantetheine is not boxed.

Stereo views of the binding of CoA to PPAT. For clarity, water molecules (red spheres) are not labeled, and only the groups that participate in binding (i.e., main chain or the side chain atoms) are shown. The protein structures shown in panels a and b are in similar orientations. Labels for residues belonging to a threefold-related subunit are italicized. Final σ_A (28) weighted difference omit electron density map for CoA bound to subunit B (a), where electron density is visible only for the Ppant moiety of CoA, and the twofold-related protomer A (b). The contour level of the electron density maps is 2.5σ, and the resolution is 1.78 Å. The bonds of CoA (pink) and the bonds of the enzyme (white) are shown. Color coding of atoms is explained in the legend to Fig. 2. This figure was produced with MolScript (19) or BobScript (11) and Raster3D (22) programs.

(a) Stereodiagram of the PPAT:CoA Cα trace of the two superimposed subunits within the asymmetric unit. Protomer A showing the entire inhibitor (magenta) and the twofold-related superimposed protomer B (teal) are shown. Ligands are shown in ball and stick representation. Oxygen (red) nitrogen (blue), sulfur (green), carbon (yellow), and phosphorus (black) atoms are shown. The overall rms deviation for the 314 equivalent Cα positions of the superposition of the two subunits in the asymmetric unit is 0.81 Å. This color coding of atoms is also used in panel b and in Fig. 3. (b) Stereodiagram of the PPAT Cα trace of the dimer within the asymmetric unit bound to CoA (blue), dPCoA (red) (Protein Data Bank identification code [PDB ID] 1B6T) (14), ATP (yellow) (PDB ID 1GN8) (16), and Ppant (green) (PDB ID 1QJC) (16). Protomer A (top subunit) binds CoA in the PPAT:CoA structure and ATP in the PPAT:ATP structure, while protomer B (bottom subunit) binds dPCoA (PPAT:dPCoA structure), ATP (PPAT:ATP structure), and Ppant (PPAT:Ppant structure) but shows only partially ordered binding to CoA (PPAT:CoA structure). This figure was produced with MolScript (19) or BobScript (11) and Raster3D (22) programs.