Preparation of antiserum against rat delta6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha

Biosci Biotechnol Biochem. 2003 May;67(5):1177-8. doi: 10.1271/bbb.67.1177.

Abstract

Anti-rat delta6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat delta6-desaturase. The antiserum prevented the enzymatic activity of delta6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha, is involved in activating delta6-desaturase gene expression, thereby elevating the protein level and the activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry*
  • Antibodies / isolation & purification
  • Gemfibrozil / pharmacology*
  • Hypolipidemic Agents / pharmacology*
  • Ligands
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / enzymology
  • Molecular Weight
  • Rabbits / immunology
  • Rats
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Stearoyl-CoA Desaturase / immunology*
  • Transcription Factors / metabolism*

Substances

  • Antibodies
  • Hypolipidemic Agents
  • Ligands
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors
  • Stearoyl-CoA Desaturase
  • delta 6-desaturase, rat
  • Gemfibrozil