Abstract
Anti-rat delta6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat delta6-desaturase. The antiserum prevented the enzymatic activity of delta6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha, is involved in activating delta6-desaturase gene expression, thereby elevating the protein level and the activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / chemistry*
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Antibodies / isolation & purification
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Gemfibrozil / pharmacology*
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Hypolipidemic Agents / pharmacology*
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Ligands
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Microsomes, Liver / drug effects
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Microsomes, Liver / enzymology
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Molecular Weight
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Rabbits / immunology
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Rats
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Stearoyl-CoA Desaturase / immunology*
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Transcription Factors / metabolism*
Substances
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Antibodies
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Hypolipidemic Agents
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Ligands
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Stearoyl-CoA Desaturase
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delta 6-desaturase, rat
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Gemfibrozil