Neuron. 2003 Jun 19;38(6):871-85.

Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system.

Source

Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.

Abstract

Axon pruning is widely used for the refinement of neural circuits in both vertebrates and invertebrates, and may also contribute to the pathogenesis of neurodegenerative diseases. However, little is known about the cellular and molecular mechanisms of axon pruning. We use the stereotyped pruning of gamma neurons of the Drosophila mushroom bodies (MB) during metamorphosis to investigate these mechanisms. Detailed time course analyses indicate that MB axon pruning is mediated by local degeneration rather than retraction and that the disruption of the microtubule cytoskeleton precedes axon pruning. In addition, multiple lines of genetic evidence demonstrate an intrinsic role of the ubiquitin-proteasome system in axon pruning; for example, loss-of-function mutations of the ubiquitin activating enzyme (E1) or proteasome subunits in MB neurons block axon pruning. Our findings suggest that some forms of axon pruning during development may share similarities with degeneration of axons in response to injury.

Comment in

Neuron. 2003 Jun 19;38(6):849-52.

PMID:: 12818174; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Grant Support

R01-NS41044/NS/NINDS NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

FlyBase

Supplemental Content

Save items

Related citations in PubMed

Engulfing action of glial cells is required for programmed axon pruning during Drosophila metamorphosis. [Curr Biol. 2004]
Engulfing action of glial cells is required for programmed axon pruning during Drosophila metamorphosis.
Awasaki T, Ito K. Curr Biol. 2004 Apr 20; 14(8):668-77.
Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning. [J Neurosci. 2008]
Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning.
Hoopfer ED, Penton A, Watts RJ, Luo L. J Neurosci. 2008 Jun 11; 28(24):6092-103.
Glia engulf degenerating axons during developmental axon pruning. [Curr Biol. 2004]
Glia engulf degenerating axons during developmental axon pruning.
Watts RJ, Schuldiner O, Perrino J, Larsen C, Luo L. Curr Biol. 2004 Apr 20; 14(8):678-84.
Review Programmed axon death, synaptic dysfunction and the ubiquitin proteasome system. [Curr Drug Targets CNS Neurol D...]
Review Programmed axon death, synaptic dysfunction and the ubiquitin proteasome system.
Coleman MP, Ribchester RR. Curr Drug Targets CNS Neurol Disord. 2004 Jun; 3(3):227-38.
Review [Human diseases associated with ubiquitin/proteasome proteolytic system]. [Tanpakushitsu Kakusan Koso. 1999]
Review [Human diseases associated with ubiquitin/proteasome proteolytic system].
Anan T, Nakao M. Tanpakushitsu Kakusan Koso. 1999 May; 44(6):776-86.

See reviews... See all...

Cited by 58 PubMed Central articles

Ubiquitin homeostasis is critical for synaptic development and function. [J Neurosci. 2011]
Ubiquitin homeostasis is critical for synaptic development and function.
Chen PC, Bhattacharyya BJ, Hanna J, Minkel H, Wilson JA, Finley D, Miller RJ, Wilson SM. J Neurosci. 2011 Nov 30; 31(48):17505-13.
Anatomy of human sensory cortices reflects inter-individual variability in time estimation. [Front Integr Neurosci. 2011]
Anatomy of human sensory cortices reflects inter-individual variability in time estimation.
Gilaie-Dotan S, Kanai R, Rees G. Front Integr Neurosci. 2011; 5:76. Epub 2011 Nov 21.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling. [PLoS One. 2011]
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ. PLoS One. 2011; 6(9):e24168. Epub 2011 Sep 8.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Axon pruning during Drosophila metamorphosis: evidence for local degeneration an...
Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system.
Neuron. 2003 Jun 19 ;38(6):871-85.

PubMed
Breast cancer patients with bone metastases are characterised by increased level...
Breast cancer patients with bone metastases are characterised by increased levels of nonisomerised type I collagen fragments.
Breast Cancer Res. 2003 ;5(4):R103-9. Epub 2003 May 19 .

PubMed
Public health surveillance for behavioral risk factors in a changing environment...
Public health surveillance for behavioral risk factors in a changing environment. Recommendations from the Behavioral Risk Factor Surveillance Team.
MMWR Recomm Rep. 2003 May 23 ;52(RR-9):1-12.

PubMed
Limitations of brain imaging in forensic psychiatry.
Limitations of brain imaging in forensic psychiatry.
J Am Acad Psychiatry Law. 2003 ;31(1):89-96.

PubMed
Evidence for specific N-terminal galanin fragment binding sites in the rat brain...
Evidence for specific N-terminal galanin fragment binding sites in the rat brain.
Eur J Pharmacol. 1992 Dec 2 ;224(2-3):203-5.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: