Kinetic parameters of continuous cultures of Bacillus thermoleovorans sp. A2 degrading phenol at 65 degrees C

J Biotechnol. 2003 Jun 26;103(2):129-35. doi: 10.1016/s0168-1656(03)00105-6.

Abstract

In this paper, we report on the kinetics of phenol degradation and cell growth in continuous cultures of suspended cells of Bacillus thermoleovorans sp. A2 at 65 degrees C. A high yield coefficient of Y(x/s)=0.84 g cell dry weight g(-1) phenol was measured at a dilution rate of 0.5 h(-1). At the same dilution rate the coefficient for maintenance metabolism (m(s)) was determined to be 0.045 g phenol g(-1) cell dry weight h(-1). The maximal growth rate (wash-out) determined at a phenol inlet concentration of 188 mg l(-1) was 0.9 h(-1). Up to 7 g phenol l(-1) per day were degraded in a continuously operated 2-l stirred tank reactor with suspended cells (feed concentration 660 mg l(-1)). Additionally, yield coefficients for oxygen and ammonium are reported.

MeSH terms

  • Bacillus / growth & development*
  • Bacillus / metabolism*
  • Biodegradation, Environmental*
  • Bioreactors / microbiology*
  • Culture Media
  • Hot Temperature
  • Kinetics
  • Minerals
  • Models, Biological
  • Oxygen / metabolism
  • Phenol / metabolism*
  • Quaternary Ammonium Compounds / metabolism
  • Waste Disposal, Fluid / methods*

Substances

  • Culture Media
  • Minerals
  • Quaternary Ammonium Compounds
  • Phenol
  • Oxygen