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BMC Blood Disord. 2003 Jun 16;3(1):1.

Fanconi anemia genes are highly expressed in primitive CD34+ hematopoietic cells.

Author information

  • 1Unité de génétique humaine et moléculaire, CHUQ-Hôpital St-François d'Assise, 10 rue de l'Espinay, Québec, Qc, Canada G1L 3L5. madeleine.carreau@crsfa.ulaval.ca

Abstract

BACKGROUND:

Fanconi anemia (FA) is a complex recessive genetic disease characterized by progressive bone marrow failure (BM) and a predisposition to cancer. We have previously shown using the Fancc mouse model that the progressive BM failure results from a hematopoietic stem cell defect suggesting that function of the FA genes may reside in primitive hematopoietic stem cells.

METHODS:

Since genes involved in stem cell differentiation and/or maintenance are usually regulated at the transcription level, we used a semiquantitative RT-PCR method to evaluate FA gene transcript levels in purified hematopoietic stem cells.

RESULTS:

We show that most FA genes are highly expressed in primitive CD34-positive and negative cells compared to lower levels in more differentiated cells. However, in CD34- stem cells the Fancc gene was found to be expressed at low levels while Fancg was undetectable in this population. Furthermore, Fancg expression is significantly decreased in Fancc -/- stem cells as compared to wild-type cells while the cancer susceptibility genes Brca1 and Fancd1/Brac2 are upregulated in Fancc-/- hematopoietic cells.

CONCLUSIONS:

These results suggest that FA genes are regulated at the mRNA level, that increased Fancc expression in LTS-CD34+ cells correlates with a role at the CD34+ differentiation stage and that lack of Fancc affects the expression of other FA gene, more specifically Fancg and Fancd1/Brca2, through an unknown mechanism.

PMID:
12809565
[PubMed - as supplied by publisher]
PMCID:
PMC194856
Free PMC Article
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