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Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):8013-8. Epub 2003 Jun 10.

Function and glycosylation of plant-derived antiviral monoclonal antibody.

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  • 1Biotechnology Foundation Laboratories at Thomas Jefferson University, 1020 Locust Street, JAH, Philadelphia, PA 19107, USA.

Abstract

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current methods of antibody production in animal systems. In this study, the heavy and light chains of human anti-rabies mAb were expressed and assembled in planta under the control of two strong constitutive promoters. An alfalfa mosaic virus untranslated leader sequence and Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal were linked at the N and C terminus of the heavy chain, respectively. mAbP was as effective at neutralizing the activity of the rabies virus as the mammalian-derived antibody (mAbM) or human rabies Ig (HRIG). The mAbP contained mainly oligomannose type N-glycans (90%) and had no potentially antigenic alpha(1,3)-linked fucose residues. mAbP had a shorter half-life than mAbM. The mAbP was as efficient as HRIG for post-exposure prophylaxis against rabies virus in hamsters, indicating that differences in N-glycosylation do not affect the efficacy of the antibody in this model.

PMID:
12799460
[PubMed - indexed for MEDLINE]
PMCID:
PMC164704
Free PMC Article
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