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Science. 2003 Jun 6;300(5625):1581-4.

Programmed DNA deletion as an RNA-guided system of genome defense.

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  • 1Division of Basic Sciences, Fred Hutchinson Cancer ResearchCenter, 1100 Fairview Avenue North, Post Office Box 19024, Seattle, WA 98109, USA. mcyao@fhcrc.org

Abstract

Genomewide DNA rearrangements occur in many eukaryotes during development, but their functions and mechanisms are poorly understood. Previous studies have implicated a sequence-recognition mechanism based on RNA-mediated interactions between nuclei in ciliated protozoa. In this study, we found that the process recognized and deleted a foreign gene integrated in a Tetrahymena chromosome, suggesting an unusual mechanism of genome surveillance. We further found that injection of double-stranded RNA into the cell at specific developmental stages triggers efficient deletion of the targeted genomic regions. Together the results indicate an RNA-based mechanism that directs genomewide DNA rearrangements and serves to disable invading genetic agents.

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PMID:
12791996
[PubMed - indexed for MEDLINE]
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