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Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7726-30. Epub 2003 Jun 3.

Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis.

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  • 1Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. sasakura@ascidian.zool.kyotou.ac.jp


The tadpole larva of the basal chordate Ciona intestinalis has the most simplified, basic body-plan of chordates. Because it has a compact genome with a complete draft sequence, a large quantity of EST/cDNA information, and a short generation time, Ciona is a suitable model for future genetics. We establish here a transgenic technique in Ciona that uses the Tc1/mariner superfamily transposon Minos. Minos was integrated efficiently into the genome of germ cells and transmitted stably to subsequent generations. In addition, an enhancer-trap line was obtained. This is a demonstration of efficient, Minos-mediated transgenesis in marine invertebrates.

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