Oxylipins and ascospore morphology in the ascomycetous yeast genus Dipodascus

D P Smith; J L F Kock; P W J van Wyk; C H Pohl; E van Heerden; P J Botes; S Nigam

doi:10.1023/a:1023340900369

Oxylipins and ascospore morphology in the ascomycetous yeast genus Dipodascus

Antonie Van Leeuwenhoek. 2003;83(4):317-25. doi: 10.1023/a:1023340900369.

Authors

D P Smith¹, J L F Kock, P W J van Wyk, C H Pohl, E van Heerden, P J Botes, S Nigam

Affiliation

¹ Lipid Biotechnology Group, Department of Microbiology, Biochemistry and Food Sciences, University of the Free State, PO Box 339, 9301 Bloemfontein, South Africa.

PMID: 12777067
DOI: 10.1023/a:1023340900369

Abstract

Immunofluorescence microscopy was used to assess members of the yeast genus Dipodascus for the presence of 3-hydroxy oxylipins. Fluorescence was associated with the aggregating ascospores in all species tested, thus suggesting the association of 3-hydroxy oxylipins with these cells, especially the surrounding slime sheaths. An ultrastructural study of the ascospores revealed sheaths with indentations, probably caused by the close packing of the ascospores to form clusters. In addition, an increase in the neutral and glycolipid fractions as well as a decrease in the phospholipid fraction during ascosporogenesis in D. ambrosiae was found.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fatty Acids, Unsaturated / metabolism*
Hydroxyeicosatetraenoic Acids / metabolism*
Microscopy, Fluorescence
Saccharomycetales / physiology*
Saccharomycetales / ultrastructure
Spores, Fungal / physiology
Spores, Fungal / ultrastructure*

Substances

Fatty Acids, Unsaturated
Hydroxyeicosatetraenoic Acids
3-hydroxy-5,8,11,14-eicosatetraenoic acid