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Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80. Epub 2003 May 19.

Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.

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  • 1Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Abstract

Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer.

PMID:
12756303
[PubMed - indexed for MEDLINE]
PMCID:
PMC165832
Free PMC Article
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