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    FEBS Lett. 2003 May 22;543(1-3):21-4.

    Identification and characterisation of human aldose 1-epimerase.

    Timson DJ, Reece RJ.

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    School of Biological Sciences, The University of Manchester, 2.205 Stopford Building, Oxford Road, M13 9PT, Manchester, UK.

    Abstract

    Aldose 1-epimerase or mutarotase (EC 5.1.3.3) is a key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha- and beta-anomers of hexose sugars such as glucose and galactose. We identified an open reading frame in the human genome (BC014916) which has high sequence similarity to previously identified bacterial aldose 1-epimerases. This sequence was cloned into a bacterial expression vector, and expressed and purified from this source. Enzyme assays show that the protein has aldose 1-epimerase activity and exhibits a preference for galactose over glucose. Site-directed mutagenesis confirmed the involvement of three residues involved in catalysis and substrate binding.

    PMID:
    12753898
    [PubMed - indexed for MEDLINE]

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