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FEBS Lett. 2003 May 22;543(1-3):16-20.

Identification of two serine residues important for p53 DNA binding and protein stability.

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  • 1Department of Biochemistry, University of California, Riverside, CA 92521, USA.

Abstract

The p53 core DNA binding domain has been implied in Mdm2-mediated protein degradation. Here we show that the substitution of the serine residues 116 and 127 with alanine residues (S116/127A) has no effect on p53 DNA binding and protein stability. However, the substitution of the serine residues with the aspartic acid (S116/127D) abolished p53 DNA binding and led to protein stabilization. Importantly, we have shown that S116/127D exhibits a structural mutant conformation that results in a loss of p53-dependent transcription and Mdm2-mediated protein degradation.

PMID:
12753897
[PubMed - indexed for MEDLINE]
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