Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genetics. 2003 May;164(1):311-21.

A bacterial artificial chromosome contig spanning the major domestication locus Q in wheat and identification of a candidate gene.

Author information

  • 1USDA-ARS Cereal Crops Research Unit, Northern Crop Science Laboratory, Fargo, North Dakota 58105, USA. justin.faris@ndsu.nodak.edu

Abstract

The Q locus played a major role in the domestication of wheat because it confers the free-threshing character and influences many other agronomically important traits. We constructed a physical contig spanning the Q locus using a Triticum monococcum BAC library. Three chromosome walking steps were performed by complete sequencing of BACs and identification of low-copy markers through similarity searches of database sequences. The BAC contig spans a physical distance of approximately 300 kb corresponding to a genetic distance of 0.9 cM. The physical map of T. monococcum had perfect colinearity with the genetic map of wheat chromosome arm 5AL. Recombination data in conjunction with analysis of fast neutron deletions confirmed that the contig spanned the Q locus. The Q gene was narrowed to a 100-kb segment, which contains an APETALA2 (AP2)-like gene that cosegregates with Q. AP2 is known to play a major role in controlling floral homeotic gene expression and thus is an excellent candidate for Q.

PMID:
12750342
[PubMed - indexed for MEDLINE]
PMCID:
PMC1462558
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk