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1: J Struct Biol. 2003 Apr;142(1):154-61.Click here to read Links

High-throughput protein crystallization.

Hui R, Edwards A.

Affinium Pharmaceuticals, 100 University Avenue, Ont., Toronto, Canada.

High-throughput structural biology is a focus of a number of academic and pharmaceutical laboratories around the world. The use of X-ray crystallography in these efforts is critically dependent on high-throughput protein crystallization. The application of current protocols yields crystal leads for approximately 30% of the input proteins and well-diffracting crystals for a smaller fraction. Increasing the success rate will require a multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation.

PMID: 12718927 [PubMed - indexed for MEDLINE]