Nat Biotechnol. 2003 May;21(5):513-8. Epub 2003 Apr 21.

Repair of bone defects using synthetic mimetics of collagenous extracellular matrices.

Lutolf MP, Weber FE, Schmoekel HG, Schense JC, Kohler T, Müller R, Hubbell JA.

Source

Institute for Biomedical Engineering, Swiss Federal Institute of Technology, Zurich and University of Zurich, Zurich, Switzerland.

Abstract

We have engineered synthetic poly(ethylene glycol) (PEG)-based hydrogels as cell-ingrowth matrices for in situ bone regeneration. These networks contain a combination of pendant oligopeptide ligands for cell adhesion (RGDSP) and substrates for matrix metalloproteinase (MMP) as linkers between PEG chains. Primary human fibroblasts were shown to migrate within these matrices by integrin- and MMP-dependent mechanisms. Gels used to deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) to the site of critical- sized defects in rat crania were completely infiltrated by cells and were remodeled into bony tissue within five weeks. Bone regeneration was dependent on the proteolytic sensitivity of the matrices and their architecture. The cell-mediated proteolytic invasiveness of the gels and entrapment of rhBMP-2 resulted in efficient and highly localized bone regeneration.

Comment in

Nat Biotechnol. 2003 May;21(5):506-8.

PMID:: 12704396; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Engineering what comes naturally. [Nat Biotechnol. 2003]
Engineering what comes naturally.
Wozney JM, Li RH. Nat Biotechnol. 2003 May; 21(5):506-8.
Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics. [Proc Natl Acad Sci U S A. 2003]
Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics.
Lutolf MP, Lauer-Fields JL, Schmoekel HG, Metters AT, Weber FE, Fields GB, Hubbell JA. Proc Natl Acad Sci U S A. 2003 Apr 29; 100(9):5413-8. Epub 2003 Apr 9.
[Tissue engineered bone regeneration of periosteal cells using recombinant human bone morphogenetic protein 2 induce]. [Zhongguo Xiu Fu Chong Jian Wai...]
[Tissue engineered bone regeneration of periosteal cells using recombinant human bone morphogenetic protein 2 induce].
Zhang C, Hu Y, Xiong Z, Zhang S, Yan Y, Cui F. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Feb; 19(2):100-4.
Review New synthetic biodegradable polymers as BMP carriers for bone tissue engineering. [Biomaterials. 2003]
Review New synthetic biodegradable polymers as BMP carriers for bone tissue engineering.
Saito N, Takaoka K. Biomaterials. 2003 Jun; 24(13):2287-93.
Review Tissue engineering: TGF-beta superfamily members and delivery systems in bone regeneration. [Expert Rev Mol Med. 2002]
Review Tissue engineering: TGF-beta superfamily members and delivery systems in bone regeneration.
Ramoshebi LN, Matsaba TN, Teare J, Renton L, Patton J, Ripamonti U. Expert Rev Mol Med. 2002 Sep 2; 4(20):1-11. Epub 2002 Sep 2.

See reviews... See all...

Cited by 38 PubMed Central articles

Elastomeric polypeptide-based biomaterials. [J Polym Sci A Polym Chem. 2010]
Elastomeric polypeptide-based biomaterials.
Li L, Charati MB, Kiick KL. J Polym Sci A Polym Chem. 2010 Oct; 1(8):1160-1170.
Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation. [Soft Matter. 2011]
Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation.
Bae H, Ahari AF, Shin H, Nichol JW, Hutson CB, Masaeli M, Kim SH, Aubin H, Yamanlar S, Khademhosseini A. Soft Matter. 2011 Jan 1; 7(5):1903-1911.
Biodegradable and biocompatible synthetic saccharide-Peptide hydrogels for three-dimensional stem cell culture. [Biomacromolecules. 2011]
Biodegradable and biocompatible synthetic saccharide-Peptide hydrogels for three-dimensional stem cell culture.
Chawla K, Yu TB, Liao SW, Guan Z. Biomacromolecules. 2011 Mar 14; 12(3):560-7. Epub 2011 Feb 8.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Repair of bone defects using synthetic mimetics of collagenous extracellular mat...
Repair of bone defects using synthetic mimetics of collagenous extracellular matrices.
Nat Biotechnol. 2003 May ;21(5):513-8. Epub 2003 Apr 21 .

PubMed
Inhibitory effect of a leukotriene receptor antagonist (montelukast) on neurokin...
Inhibitory effect of a leukotriene receptor antagonist (montelukast) on neurokinin A-induced bronchoconstriction.
J Allergy Clin Immunol. 2003 Apr ;111(4):833-9.

PubMed
The three-dimensional structure of human splenic white pulp compartments.
The three-dimensional structure of human splenic white pulp compartments.
J Histochem Cytochem. 2003 May ;51(5):655-64.

PubMed
Specific gene expression of ATP-binding cassette transporters and nuclear hormon...
Specific gene expression of ATP-binding cassette transporters and nuclear hormone receptors in rat liver parenchymal, endothelial, and Kupffer cells.
J Biol Chem. 2003 Jul 11 ;278(28):25448-53. Epub 2003 Apr 18 .

PubMed
Antigenic and biological characteristics of mutant strains of Cryptococcus neofo...
Antigenic and biological characteristics of mutant strains of Cryptococcus neoformans lacking capsular O acetylation or xylosyl side chains.
Infect Immun. 2003 May ;71(5):2868-75.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: