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J Am Chem Soc. 2003 Apr 23;125(16):4708-9.

A fluorogenic dye activated by the staudinger ligation.

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  • 1Center for New Directions in Organic Synthesis, Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.


Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.

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