Dark adaptation curves were determined for an amblyopic and a normal control subject following a variety of preadaptation conditions. Sizable between-eye differences were found in the dark adaptation functions of only the amblyope; the magnitude of such differences varied with this subject's distance from the light preadapting screen and with the effective size of his pupils during the light preadaptation period. Marked differences were also found between the dark adaptation functions of both eyes of the amblyopic subject and those of the normal subject. The preadaptation-dependent changes in the dark adaptation function of the amblyopic subject are hypothesized to result from anomalies of amblyopic eye light adaptation which are referable to disturbances of retinal receptor alignment.