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Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5097-102. Epub 2003 Apr 11.

Activation of archaeal transcription by recruitment of the TATA-binding protein.

Author information

  • 1Center for Molecular Genetics and Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA. mouham@biomail.ucsd.edu

Abstract

The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type transcription machinery from an upstream activating region composed of two Ptr2-binding sites. Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter.

PMID:
12692306
[PubMed - indexed for MEDLINE]
PMCID:
PMC154304
Free PMC Article
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