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Dev Genes Evol. 2003 May;213(4):193-8. Epub 2003 Apr 11.

Construction of a backcross progeny collection of dextral and sinistral individuals of a freshwater gastropod, Lymnaea stagnalis.

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  • 1Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, 153-8902, Tokyo, Japan.

Abstract

The handedness of gastropods is genetically determined, but the molecular nature of the gene responsible remains unknown. In order to identify the gene by a forward genetic approach, we carried out backcross breeding between dextral and sinistral inbred strains of a freshwater gastropod, Lymnaea stagnalis, cultivated in the laboratory. We used the dextral animals as donor "fathers" and the sinistral animals as recurrent "mothers". Each of the backcross progeny obtained was typed for the chirality of the next generation offspring oviposited by it, because the genotype for the handedness locus emerges as filial chirality. We constructed a collection of DNA and RNA specimens that included about 200 of the backcross F(2) progeny. Success in breeding was confirmed by the expected inheritance behavior of strain-specific DNA markers in the progeny.

[PubMed - indexed for MEDLINE]
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